lactate oxidase enzyme  (LABTEST DIAGNOSTICA)

Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Lactate oxidation reaction. The reaction shows the oxidation of lactate to pyruvate by lactate oxidase (LOx) in the presence of oxygen (O 2 ), generating hydrogen peroxide (H 2 O 2 ) as a byproduct.

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques:

Journal: RSC Advances

Article Title: Electrochemical evaluation of screen-printed sensor manufacturing and LOx enzyme immobilization for lactate biomarker detection: influence of reference electrode material

doi: 10.1039/d4ra06473a

Figure Lengend Snippet: Review of printed sensors for enzymatic detection of the lactate analyte. NR: not reported. HRP: horseradish peroxidase

Article Snippet: Nafion 117 (5%, Sigma-Aldrich) and Lactate Oxidase (LOx) Enzyme from a commercial kit (Labtest® Kit) suspended in 50 mmol per L buffer pH 7.2, 4-aminoantipyrine ≥ 0.05 mmol per L peroxidase ≥ 1000 U L −1 and preservatives, lactate oxidase ≥ 1000 U L −1 , N -ethyl- N -(2-hydroxy-3-sulfopropyl)-3-methyllaniline (TOOS) ≥ 1.5 mmol L −1 and preservatives were used to modify the sensors.

Techniques: Modification, Encapsulation, Membrane

Journal: Biomaterials Research

Article Title: Targeted H 2 S-Mediated Gas Therapy with pH-Sensitive Release Property for Myocardial Ischemia–Reperfusion Injury by Platelet Membrane

doi: 10.34133/bmr.0061

Figure Lengend Snippet: (A) Schematic diagram of H/R myocardial cell model building. (B) Cell viability of H/R H9C2 cells of different periods of hypoxia. (C) Cellular viability of H/R myocardial cells after different treatments. (D) Flow cytometry profiles of apoptotic H/R cardiomyocytes after different treatments. (E) ELISA experimental procedure flowchart. Cardiac levels of CK-MB (F) and LDH (G) in H/R cardiomyocytes. Extracellular cytokine levels of TNF-α (H), IL-1β (I), IL-6 (J), and IL-10 (K) in H/R cardiomyocytes. Statistical analysis was performed using one-way ANOVA. When *** P < 0.001, ** P < 0.01, and * P < 0.05, the difference was statistically significant. Data were shown as means ± SD ( n = 3).

Article Snippet: Mouse lactic dehydrogenase (LDH) enzyme-linked immunosorbent assay (ELISA) kit, mouse creatine kinase isoenzyme (CK-MB) ELISA kit, mouse interleukin-10 (IL-10) ELISA kit, mouse IL-6 ELISA kit, mouse tumor necrosis factor-α (TNF-α) ELISA kit, and mouse IL-1β ELISA kit were bought by Elabscience Biotechnology Co. Ltd. (Wuhan, China).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Biomaterials Research

Article Title: Targeted H 2 S-Mediated Gas Therapy with pH-Sensitive Release Property for Myocardial Ischemia–Reperfusion Injury by Platelet Membrane

doi: 10.34133/bmr.0061

Figure Lengend Snippet: (A) ELISA experimental procedure flowchart. Cardiac levels of CK-MB (B) and LDH (C) in the serum of MIRI mice. Cytokine levels of TNF-α (D), IL-1β (E), IL-6 (F), and IL-10 (G) in the serum of MIRI mice. (H) H&E staining and (I) Masson staining of MIRI mice treated with different formulations. Scale bar, 200 nm. Statistical analysis was performed using one-way ANOVA. When *** P < 0.001, ** P < 0.01, and * P < 0.05, the difference was statistically significant. Data were shown as means ± SD ( n = 3).

Article Snippet: Mouse lactic dehydrogenase (LDH) enzyme-linked immunosorbent assay (ELISA) kit, mouse creatine kinase isoenzyme (CK-MB) ELISA kit, mouse interleukin-10 (IL-10) ELISA kit, mouse IL-6 ELISA kit, mouse tumor necrosis factor-α (TNF-α) ELISA kit, and mouse IL-1β ELISA kit were bought by Elabscience Biotechnology Co. Ltd. (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Staining